Fluorescence microscopy

Instrumental Solutions

11-micrometer polystyrene beads stained with Nile red

Sub-resolution detection of
Nile red stained nanoparticles

70-nm polystyrene beads dried on slide

Beads aggregate at edge
FOV: 0.35 x 0 .56 mm

70-nm polystyrene beads in water under a coverslip

Beads exhibit little aggregation
FOV: 0.35 x 0 .56 mm

70-nm Isolated Polystyrene Bead

Single polystyrene nanoparticle (from image above left)
Orange filter OG530;
FLIR Blackfly 2.3 MP color camera, 4.2 fps;
450 nm excitation;
Magnification: 20X
NA: 0.42
Pixel size: 300 nm
Optical Resolution: 700 nm

We recently collaborated with local environmental scientists on a study of fluorescent dyes for MNP detection. DCVJ and Nile red dyes were compared. (1)

(1) The comparative strengths and limitations of Nile Red and 9-(dicyanovinyl)-julolidine (DCVJ) fluorescent dyes for detecting microplastics and nanoplastics
Madison Wallner, Justin Diaz, Amelia B Labbe, James J Jacob, Quentin Williams, Adina Paytan, Clive R Bagshaw
bioRxiv 2026.08.03.742549; doi: https://doi.org/10.64898/2026.08.03.742549
This article is a preprint and has not been certified by peer review